Figure 4.

Positional Epitope Coverage of Mosaic recombinant antigen. (A) 9-mer coverage by position. (B) Ranked 9-mer coverage. The results indicated epitope coverage over the position of (a) H1m-H1; (b) H1m-H3; (c) H1m-Vic; and (d) H1m-Yam. Matched 9-mers compared to upper bounds (ranked): Proportion of 9-mers in the test set “the HA amino acid sequences of seasonal influenza virus vaccine strains from 2009 to 2021” that is exactly and approximately matched in the antigen/peptide set “Mosaic recombinant antigen,” based on a sliding window across the alignment. Each x-value represents a column in the multiple sequence alignment, ranked from left to right by exact-match proportion (descending); the y-values represent proportions of the background sequences at that position which have a 9-mer that is matched at different levels in the sequences in the antigen/peptide set. Exact match proportions are shown in red, 1-off (8/9) in orange and 2-off (7/9) in yellow.