Table 1.
Bacterial strains, plasmids and primers used for this study
|
Strain/plasmid/primer (5′−3′) |
Relevant characteristics |
Source or reference |
|---|---|---|
|
E. coli INV110 |
For growth and purification of plasmid DNA digested with dam or dcm methylation-sensitive restriction enzymes |
Invitrogen |
|
E. coli TOP10 |
General cloning and blue/white screening |
Life Technologies |
|
E. coli S17-1 |
Mobilizing strain, transfer genes of RP4 integrated in chromosome; Smr |
[51] |
|
pMo130 |
Kmr, Burkholderia gene replacement vector |
[34] |
|
pBHR2 |
Broad-host-range plasmid, Kmr |
[35] |
|
pBHR2-BPSS2185 |
pBHR2 containing a full-length copy of BPSS2185 |
This study |
|
B. pseudomallei JW270 |
Strain excluded from the CDC select agent list as a result of a deletion of the wcb locus encoding the 6-deoxyheptan capsular polysaccharide; ∆(amrR-oprA) rpsL (Smr) |
[52] |
|
B. pseudomallei JW270 ΔBPSS2185 |
JW270 derivative with an in-frame 138 bp deletion of BPSS2185 |
This study |
|
BPSS2185 F1 |
*TTAT CATATG GAATACCGGGGTAA |
This study |
|
BPSS2185 R1 |
GAGGCTGGACATGACT |
This study |
|
BPSS2185 F2 |
AGCCTCACGATCGGCAACGA |
This study |
|
BPSS2185 R2 |
*TGTA GGATCC GAGCGGAAAGCAT |
This study |
|
C-BPSS2185 F |
*TTA CATATG ACGGCAAGGGTTTTA |
This study |
|
C-BPSS2185 R |
*TATA GGATCC ACGCACCCTTACA |
This study |
Kmr, kanamycin resistant; Smr, streptomycin resistant.
*Restriction enzyme sites are bolded, italicized and underlined. CATATG – NdeI, GGATCC – BamHI.