Table 2.
Effect of a gadE mutation and complementation of the mutation on type 1 pilus production in E. coli measured by the hemagglutination (HA) titre in a K-12 strain and a uropathogenic clinical isolate
|
HA Titre |
||||
|---|---|---|---|---|
|
Strain |
pH 5.5 |
pH5.5+* |
pH 7.0 |
pH 7.0+* |
|
EK227 |
64 |
32 |
256 |
128 |
|
EF1007 (gadE) |
16 |
16 |
256 |
128 |
|
EF1083 (gadE/pPCRScript gadE) |
64 |
32 |
256 |
128 |
|
NU149 |
256† |
64 |
1024 |
256 |
|
NU149 gadE |
128 |
32 |
1024 |
256 |
|
NU149 gadE/pPCRScript gadE |
256 |
64 |
1024 |
256 |
*+=addition of 400 mM NaCl.
†Mean value from at least three separate runs.