FIG. 5.

(A) relA-independent stimulation of full-length transcription of the fas operon following isoleucine and valine deprivation [-(I+V)] of cells. Control cells were incubated in complete CDM (+AA). The gene-specific probes used are indicated on the respective Northern blots. The ethidium bromide (EtBr)-stained gel demonstrates equal loading of the slots, integrity of the RNA, and association of the probe DNAs (fasB, fasC, and fasX) to various extents with 23S and 16S rRNA (asterisks). The indicated sizes of the hybridizing RNA species are consistent with the fas operon map shown in panel B.