TABLE 1.
Primers used to generate gene-specific PCR-derived probes from S. pyogenes NZ131 for RNA hybridizations
| Forward (F) or reverse (R) primer | SF370 genome coordinatesa |
|---|---|
| valSF | 1294547–1294566(C)b |
| valSR | 1293632–1293653 |
| ileSF | 1245196–1245218(C) |
| ileSR | 1243785–1243806 |
| ssrAF | 1065040–1065061 |
| ssrAR | 1065340–1065360(C) |
| smpBF | 406216–406235 |
| smpBR | 406617–406636(C) |
| clpPF | 334821–334843 |
| clpPR | 335341–335364(C) |
| clpCF | 1726643–1726663(C) |
| clpCR | 1725608–1725629 |
| hrcAF | 1460765–1460785(C) |
| hrcAR | 1460034–1460056 |
| dnaKF | 1459046–1459067(C) |
| dnaKR | 1457906–1457927 |
| dnaJF | 1456996–1457016(C) |
| dnaJR | 1455985–1456006 |
| groESF | 1724561–1724580(C) |
| groESR | 1724321–1724341 |
| groELF | 1723849–1723870(C) |
| groELR | 1722886–1722906 |
| sagAF | 598709–598731 |
| sagAR | 598972–598994(C) |
| fasBF | 210435–210457 |
| fasBR | 211345–211366(C) |
| fasCF | 211634–211658 |
| fasCR | 212514–212537(C) |
| fasXF | 213654–213675 |
| fasXR | 213955–213975(C) |
| luxSF | 1361940–1361962 |
| luxSR | 1362312–1362334(C) |
a
GenBank accession number AE004092.
b
C indicates the complementary strand.