Figure 4.

The in vitro determination of bacterial proteins’ immunoreactivity tested with human allergic serum. (a) SDS PAGE separation of bacterial proteins’ isolates; (b) IgG-immunoreactivity with IRDye^® 800 CW; (c) IgE-immunoreactivity with IRDye^® 680 RD; (d) Signal of immunoreactive bands normalized for each lane by deriving the ratio of the signals of the total protein in each lane. MW—molecular weight marker; CM1-CM3—proteins of bacteria isolated from cultures based on cows’ milk microbial isolates; HM1-HM3—proteins of bacteria isolated from cultures based on human milk microbial isolates. Asterisks denote significant difference with respect to the Duncan test values of immunoreactivity for the proteins isolated from microorganisms from different milk types.