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Optimal temperature of the RT-LAMP assay for PEDV. The same reaction mixtures were incubated at different temperatures (55 °C, 55.5 °C, 56.2 °C, 57.1 °C, 58.5 °C, 59.7 °C, 60.6 °C, 61.9 °C, 63.1 °C, 64.1 °C, 64.7 °C and 65 °C). The results were detected by 2% (w/v) agarose gel electrophoresis. Lane M: DL2000 DNA marker; Lane N: negative control.
