Table 1.
Sequences of primers used for RT-LAMP and RT-PCR analysis.
| Primer | Type | Position (nt) 1 | Sequence (5′-3′) |
|---|---|---|---|
| PEDV F3 | forward outer primer | 842–859 | GGAGGAGAATTCCCAAGG |
| PEDV B3 | reverse outer primer | 1022–1039 | AAGAGTCCGCTAGCTCAC |
| PEDV FIP | forward inner primer (F2-F1c) |
F1c: 900–924 F2: 860–877 |
TTCCGCATCTCCAAAATTTTTGAAG GCGAAAATAGCGTAGCAG |
| PEDV BIP | reverse inner primer (B1c-B2) |
B1c: 939–959 B2: 1002–1019 |
TGTTGATGCCTCAGGCTATGC ACAGCCACATTACCACCA |
| PEDV LF | Loop forward primer | 879–895 | CCCTGGGTCCGAAGCAA |
| PEDV LB | Loop outer primer | 975–993 | AGCACCAAATGTTGCAGCA |
1 Gene position of each primer in the nucleotide sequence of PEDV N gene (GenBank No. JN825712.1). nt: nucleotide.