Table 3.
Thawing Temp. (°C) | Modifications | VCL | VSL | VAP | LIN | STR | WOB | ALH | BCF |
---|---|---|---|---|---|---|---|---|---|
37 | Control | 73.5 ± 4.5 bcd | 53.3 ± 4 ab | 63.6 ± 4 abc | 72.5 ± 3.9 a | 83.8 ± 3.5 ab | 86.5 ± 1.3 a | 2.28 ± 0.1 cd | 8.1 ± 0.6 ab |
CLC | 67.5 ± 6.1 d | 48 ± 6.4 bc | 57.9 ± 6 c | 71.1 ± 7.5 ab | 82.8 ± 5 abc | 85.7 ± 4.3 ab | 2.22 ± 0.25 cd | 7.35 ± 0.8 cd | |
Basic pH | 67.9 ± 2.3 d | 45.5 ± 4.2 c | 57.6 ± 3.4 c | 67 ± 5.9 bc | 78.9 ± 5.3 cd | 84.8 ± 3.2 ab | 2.18 ± 0.13 d | 8.3 ± 0.4 a | |
EGTA/EDTA | 72.1 ± 5.9 cd | 52.2 ± 5.3 ab | 61.4 ± 5.9 bc | 72.4 ± 3.7 a | 85.2 ± 3 a | 85 ± 1.9 ab | 2.35 ± 0.12 bcd | 7.9 ± 0.6 ab | |
70 | Control | 79.7 ± 9.5 ab | 55.2 ± 4.6 a | 67.8 ± 8.1 ab | 69.6 ± 4.5 ab | 81.7 ± 3.9 abc | 85.1 ± 2.3 ab | 2.32 ± 0.25 ab | 8.2 ± 0.2 ab |
CLC | 72.8 ± 7.1 bcd | 49.4 ± 4.6 bc | 61.2 ± 5.3 bc | 68.1 ± 6.5 abc | 80.7 ± 4.8 bc | 84.2 ± 3.2 ab | 2.4 ± 0.29 bcd | 7.1 ± 0.4 d | |
Basic pH | 75.9 ± 4.8 abc | 48.5 ± 3.6 bc | 63.9 ± 4.8 abc | 63.9 ± 2.3 c | 75.8 ± 2.6 d | 84.2 ± 1.7 ab | 2.43 ± 0.13 abc | 8.2 ± 0.3 ab | |
EGTA/EDTA | 82.5 ± 8.3 a | 68.8 ± 7.2 a | 68.5 ± 7.7 a | 68.5 ± 4.5 abc | 82.2 ± 3.4 abc | 83.3 ± 2.5 b | 2.65 ± 0.18 a | 7.8 ± 0.7 bc |
VCL: curvilinear velocity (µm/s), VSL: straightline velocity (µm/s), VAP: average path velocity (µm/s), LIN: linearity (%), STR: straightness (STR, %), WOB: wobble (%), ALH: mean amplitude of lateral head displacement (mm), BCF: means of the beat cross frequency (Hz). Data are expressed as mean ± standard deviation. a, b, c, d indicate significant differences between treatments (p < 0.05).