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. 2022 Sep 28;12(19):2600. doi: 10.3390/ani12192600

Table 3.

Evaluation of different modifications in the thawing extenders: effect on kinematic parameters.

Thawing Temp. (°C) Modifications VCL VSL VAP LIN STR WOB ALH BCF
37 Control 73.5 ± 4.5 bcd 53.3 ± 4 ab 63.6 ± 4 abc 72.5 ± 3.9 a 83.8 ± 3.5 ab 86.5 ± 1.3 a 2.28 ± 0.1 cd 8.1 ± 0.6 ab
CLC 67.5 ± 6.1 d 48 ± 6.4 bc 57.9 ± 6 c 71.1 ± 7.5 ab 82.8 ± 5 abc 85.7 ± 4.3 ab 2.22 ± 0.25 cd 7.35 ± 0.8 cd
Basic pH 67.9 ± 2.3 d 45.5 ± 4.2 c 57.6 ± 3.4 c 67 ± 5.9 bc 78.9 ± 5.3 cd 84.8 ± 3.2 ab 2.18 ± 0.13 d 8.3 ± 0.4 a
EGTA/EDTA 72.1 ± 5.9 cd 52.2 ± 5.3 ab 61.4 ± 5.9 bc 72.4 ± 3.7 a 85.2 ± 3 a 85 ± 1.9 ab 2.35 ± 0.12 bcd 7.9 ± 0.6 ab
70 Control 79.7 ± 9.5 ab 55.2 ± 4.6 a 67.8 ± 8.1 ab 69.6 ± 4.5 ab 81.7 ± 3.9 abc 85.1 ± 2.3 ab 2.32 ± 0.25 ab 8.2 ± 0.2 ab
CLC 72.8 ± 7.1 bcd 49.4 ± 4.6 bc 61.2 ± 5.3 bc 68.1 ± 6.5 abc 80.7 ± 4.8 bc 84.2 ± 3.2 ab 2.4 ± 0.29 bcd 7.1 ± 0.4 d
Basic pH 75.9 ± 4.8 abc 48.5 ± 3.6 bc 63.9 ± 4.8 abc 63.9 ± 2.3 c 75.8 ± 2.6 d 84.2 ± 1.7 ab 2.43 ± 0.13 abc 8.2 ± 0.3 ab
EGTA/EDTA 82.5 ± 8.3 a 68.8 ± 7.2 a 68.5 ± 7.7 a 68.5 ± 4.5 abc 82.2 ± 3.4 abc 83.3 ± 2.5 b 2.65 ± 0.18 a 7.8 ± 0.7 bc

VCL: curvilinear velocity (µm/s), VSL: straightline velocity (µm/s), VAP: average path velocity (µm/s), LIN: linearity (%), STR: straightness (STR, %), WOB: wobble (%), ALH: mean amplitude of lateral head displacement (mm), BCF: means of the beat cross frequency (Hz). Data are expressed as mean ± standard deviation. a, b, c, d indicate significant differences between treatments (p < 0.05).