FIG. 5.

(A) Nucleotide sequence of the 5′ leader region of B. subtilis yuiG. Symbols: thin arrow, putative sigma A promoter sequence (ς); heavy underlines, −35 and −10 contact regions, the RBS, and the biotin regulatory region (bioO); heavy arrow, 5′ coding region of yuiG. (B) Consensus sequence of possible biotin regulatory regions. The 5′ leader regions of E. coli bioABFCD, B. subtilis bioWAFDBI-ytbQ (BSUBIO), B. subtilis yuiG (BSUYUIG), and B. sphaericus bioDAYB (BSPHBIO) were aligned by using the MegAlign algorithm of DNASTAR software. Conserved nucleotides are boxed.