TABLE 2.

Comparison of differential gene expression levels determined by real-time RT-PCR and microarray hybridization

Expt and gene	Fold change in transcript levelsa as determined by:
	RT-PCR	Microarray
+/− riboflavin treatment
ribG	3	2.0
thiA	NC	NC
bioA	NC	NC
gap	NC	NC
+/− biotin treatment
ribG	NC	NC
thiA	NC	NC
bioA	8	>100
gap	NC	NC
+/− thiamine treatment
ribG	NC	NC
thiA	33	62
bioA	NC	NC
gap	NC	NC

^aTriplet real-time RT-PCRs were performed on cDNA prepared from total RNA isolated from cells grown to exponential phase in minimal medium in the presence or absence of riboflavin (200 μg/ml), biotin (0.1 μg/ml), or thiamine (0.34 μg/ml) as described in Materials and Methods. Values presented were calculated by dividing the average fold change from untreated cells by those from vitamin-treated cells. The fold change values determined by microarray analysis were taken from Table 1 for comparison. NC, no change in real-time RT-PCR values.