TABLE 3.
Change in transcript levels of several highly expressed genes from exponential to stationary growth phase
| Gene | Enzyme and/or functionsa | Ratiob |
|---|---|---|
| acoA | Acetoin dehydrogenase E1 component (alpha subunit) | <0.01 |
| acoC | Acetoin dehydrogenase E2 component | 0.01 |
| gapB | Glyceraldehyde-3-phosphate dehydrogenase | 0.013 |
| glvC | PTS arbutin-like enzyme IIBC component | <0.01 |
| kbl | 2-Amino-3-ketobutyrate CoA ligase | 0.014 |
| licB | PTS lichenan-specific enzyme IIB component | 0.015 |
| licC | PTS lichenan-specific enzyme IIC component | 0.011 |
| pckA | Phosphoenolpyruvate carboxykinase | 0.04 |
| rbsA | Ribose ABC transporter (ATP-binding protein) | 0.016 |
| rbsD | Ribose ABC transporter (membrane protein) | 0.019 |
| tdh | Threonine 3-dehydrogenase | 0.038 |
| yjdE | Unknown; similar to mannose-6-phosphate isomerase | 0.023 |
| yusJ | Unknown; similar to butyryl-CoA dehydrogenase | 0.011 |
| yvdF | Unknown; similar to glucan 1,4-alpha-maltohydrolase | 0.011 |
| cspB | Cold shock protein | 13.8 |
| cspD | Cold shock protein | 9.7 |
| gap | Glyceraldehyde-3-phosphate dehydrogenase | 66.3 |
| lctE | l-Lactate dehydrogenase | 9.4 |
| lctP | l-Lactate permease | >100 |
| pdhA | Pyruvate dehydrogenase (E1 α subunit) | 14.3 |
| pdhB | Pyruvate dehydrogenase (E1 β subunit) | 18.3 |
| rplJ | Ribosomal protein L10 (BL5) | 91.5 |
| rspD | Ribosomal protein S4 (BS4) | 72.0 |
a
CoA, coenzyme A.
b
The repression or derepression ratios were calculated by dividing the average difference values (after normalization) from duplicate hybridization experiments of cells grown to exponential phase in minimal medium without vitamin treatment by those grown to stationary phase. See text for details. For most genes (indicated in boldface), average difference values were obtained from duplicate probe sets per microarray.