Table 4.

Antibiotic susceptibility of Q112-resistant strains.

Antibiotic	MIC (μM) a						MOA
	WT	A3 tatB	A6 tatB	B1 tatC	B2 tatC	B3 tatA
Q112	3.13	12.5	12.5	12.5	12.5	12.5	unknown
Ampicillin	>50	3.1	3.1	1.6	2.3	3.1	cell wall biosynthesis
Ampicillin + clavulanate	2.3	0.39	0.78	0.2	0.39	0.78	cell wall biosynthesis
Meropenem	1.2	0.39–0.6	0.78	0.39–0.6	0.39	0.78	cell wall biosynthesis
Meropenem + clavulanate	0.39	0.2	0.2	0.15	0.2	0.39	cell wall biosynthesis
Imipenem	0.39	0.6	0.78	0.39	0.39	6.2	cell wall biosynthesis
Imipenem + clavulanate	0.2	0.2	0.1	0.1	0.15	1.6	cell wall biosynthesis
Rifampicin	0.012	0.004	0.004	0.002	0.004	0.0028	RNA polymerase
Streptomycin	0.46	0.46	0.46	0.46	0.46	0.46	protein synthesis
Capreomycin	0.78	0.78	1.2	0.78	1.2	0.78	protein synthesis
Linezolid	0.78	2.3	2.3	2.3	2.3	1.56	protein synthesis
Moxifloxacin	0.12	0.12	0.16	0.12	0.16	0.12	DNA gyrase, topoisomerase
Bedaquiline	0.31	0.46	0.63	0.46	0.46	0.46	ATP synthesis
Clofazimine	0.16	0.16	0.24	0.12	0.24	0.63	ATP synthesis
Delamanid	0.0031	0.038	0.0092	0.019	0.019	0.0092	mycolic acid biosynthesis
Isoniazid	0.31	0.12	0.078	0.16	0.12	0.16	mycolic acid biosynthesis
Ethionamide	0.3	0.15	0.15	0.15	0.15	0.15	mycolic acid biosynthesis
Ethambutol	1.6	3.1	2.4	2.4	3.12	3.1	arabinogalactan biosynthesis
p-Aminosalicylic acid	0.3	0.39	0.6	0.3	0.3	0.6	folic acid biosynthesis and cell wall biosynthesis
D-Cycloserine	4.7	12	9.4	9.4	9.4	12	cell wall biosynthesis
Vancomycin	5	1.2	1.2	0.92	0.92	1.2	cell wall biosynthesis

^aMICs for Q112-resistant strains were determined after two weeks. MOA, mechanism of action. The gene mutated in each strain is listed below the strain designation.