Figure 2.

Oxostephanine inhibits the formation and growth of OVCAR-8 spheroids. (A) Spheroid formation in the presence of the compounds. (B) The spheroid volume was reduced following incubation with the compounds. (C) The growth of tumor spheroids was prevented by the two types of treatment: The addition of the compound at the spheroid preparation (pre) and after spheroid formation (post). The days were counted from the time of transferring the spheroid from the hanging drop to the agar plates (day 1, etc.). (D) The morphology of spheroids of cells treated under the two conditions mentioned above. Pre, compounds were added at the time of spheroid preparation; post, compounds were added and maintained in the medium for spheroid growth in the agar plate. Scale bars, 100 µm. ^*P<0.05 and ^**P<0.01, vs. control. Oxo, oxostephanine; VX, VX-680.