Table 1. Sperm penetration of pig oocytes inseminated in vitro with spermatozoa cryopreserved with different concentrations of carboxylated poly-L-lysine (CPLL) a.
| Concentration of CPLL (%) | No. of oocytes |
|||||
|---|---|---|---|---|---|---|
| Examined | Matured (mean % ± SEM) b |
Penetrated (mean % ± SEM) c |
With male and female pronuclei (mean % ± SEM) d |
Polyspermic penetrated (mean % ± SEM) d |
Monospermic with one male pronucleus and one female pronucleus (mean % ± SEM) d |
|
| 0 | 45 | 35 (77.5 ± 6.7) | 27 (77.1 ± 1.1) | 24 (89.6 ± 6.5) | 6 (20.2 ± 10.3) | 18 (69.4 ± 16.3) |
| 0.125 | 45 | 34 (75.9 ± 5.1) | 25 (73.5 ± 0.8) | 23 (91.7 ± 4.2) | 5 (20.8 ± 11.0) | 18 (70.8 ± 15.0) |
| 0.25 | 43 | 36 (83.8 ± 1.9) | 27 (75.6 ± 7.7) | 26 (96.3 ± 3.7) | 5 (19.4 ± 10.0) | 21 (76.9 ± 12.9) |
| 0.5 | 40 | 32 (79.0 ± 4.7) | 24 (74.7 ± 4.4) | 22 (93.0 ± 3.5) | 5 (24.4 ± 12.4) | 17 (68.5 ± 10.8) |
a Experiments were repeated three times. b Percentage per oocytes examined. c Percentage per oocytes matured. d Percentage per oocytes penetrated.