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. 2022 Jul 29;68(5):318–323. doi: 10.1262/jrd.2022-046

Table 4. Overlapped upstream regulators predicted from UECs and in vivo developed blastocysts.

Name Categories SOV+AI Score VIVO Score
actinomycin D biologic drug Inhi. –2.48 Inhi. –2.55
selenomethylselenocysteine chemical - endogenous mammalian Inhi. –2.24 Inhi. –2.45
LY294002* chemical drug Inhi. –2.93 Inhi. –2.71
sirolimus chemical drug Inhi. –2.35 Inhi. –5.07
U0126* chemical drug Inhi. –2.25 Inhi. –2.72
NR4A1 ligand-dependent nuclear receptor Inhi. –2.34 Inhi. –2.04
miR-29b-3p* mature microRNA Inhi. –2.91 Inhi. –2.42
miR-199a-5p* mature microRNA Inhi. –2.49 Inhi. –2.36
let-7* microRNA Inhi. –4.44 Inhi. –2.18
RICTOR other Inhi. –5.90 Inhi. –5.42
BTNL2 transmembrane receptor Inhi. –2.33 Inhi. –2.14
hydrogen peroxide chemical - endogenous mammalian Acti. 3.88 Acti. 4.12
phenylephrine chemical drug Acti. 2.24 Acti. 2.39
dalfampridine chemical drug Acti. 2.42 Acti. 3.61
cisplatin chemical drug Acti. 2.65 Acti. 2.19
lipopolysaccharide chemical drug Acti. 3.13 Acti. 2.22
daidzein chemical drug Acti. 2.36 Inhi. –2.84
forskolin chemical toxicant Acti. 2.03 Acti. 2.53
thioacetamide* chemical toxicant Acti. 2.67 Acti. 2.95
LIF cytokine Acti. 3.01 Acti. 3.26
IL6 cytokine Acti. 3.37 Acti. 3.37
AGTR2 G-protein coupled receptor Acti. 2.21 Acti. 2.21
Tgf beta group Acti. 2.68 Acti. 2.77
ERK* group Acti. 3.44 Acti. 2.70
Insulin group Acti. 4.55 Acti. 2.33
FGF2 growth factor Acti. 2.09 Acti. 2.16
ANGPT2* growth factor Acti. 2.34 Acti. 3.16
TGFB1 growth factor Acti. 2.87 Acti. 3.13
EGF growth factor Acti. 2.96 Acti. 2.74
GDF2* growth factor Acti. 3.22 Acti. 3.28
VGLL3 other Acti. 2.00 Acti. 2.45
F2* peptidase Acti. 3.34 Acti. 2.63
SMAD2 transcription regulator Acti. 2.08 Acti. 2.54
SMAD4 transcription regulator Acti. 2.52 Acti. 3.28
TP53 transcription regulator Acti. 3.33 Acti. 2.01
YAP1* transcription regulator Acti. 3.37 Acti. 3.12
STAT3 transcription regulator Acti. 4.18 Acti. 3.04
ATF4* transcription regulator Acti. 4.36 Acti. 2.57
IRF2 transcription regulator Acti. 2.02 Inhi. –2.12
SPI1 transcription regulator Acti. 2.22 Inhi. –3.22
IGF1R* transmembrane receptor Acti. 2.71 Acti. 2.01

SOV+AI, Uterine epithelial cells of super ovulated and artificially inseminated cows; VIVO, in vivo developed blastocysts; Acti, Activated upstream regulators (Acti); Inhi, Inhibited upstream regulators (Inhi). * Prediction of the molecules containing bias factors.