Figure 6.

A set of five hub genes that correlate strongly with ALSFRS-R can be used to cluster samples into groups that correspond to their ALSFRS-R scores. (A) Heatmap showing Z-scores of normalized expression values of the five genes chosen as ‘markers’ of ALSFRS-R score, along with aggregated average expression values of all five and ALSFRS-R score for each sample. (B) Dendograms created by hierarchical clustering of ALSFRS-R score on the left, and average expression of the five marker genes on the right, for vehicle samples (left) and EH301 samples (right). Central gray lines show matching of each sample over the two trees, and colored lines in the tree denote matching clusters. P-value for Baker’s gamma index calculated using 100-fold permutation.