Figure 1.

Nrx-1 mutants have decreased resistance to environmental stressors, such as starvation stress and heat stress. (A) Survival curve of Nrx-1 mutants (red and blue lines) and wild-type iso31Bw- controls (black line) during starvation. Log-rank (Mantel–Cox) test with Bonferroni-corrected significance threshold for multiple comparisons indicated that Nrx-1^273/273 and Nrx-1^241/241 mutant flies die significantly faster than wild-type flies when on media containing 0% sucrose and 2% agar. Nrx-1^273/273 and Nrx-1^241/241 were not significantly different from each other in starvation resistance. Sample number (N) per genotype: iso31Bw- N = 64, Nrx-1^273/273 N = 61, Nrx-1^241/241 N = 58. (B) Survival curve of each genotype 24 h after an acute HS of exposure to 37°C for 1 h. Log-rank (Mantel-Cox) test with Bonferroni-corrected significance threshold for multiple comparisons results indicated that Nrx-1^273/273 and Nrx-1^241/241 mutant flies have significantly reduced resistance to HS compared to wild-type flies. Additionally, Nrx-1^241/241 flies were more sensitive to HS than Nrx-1^273/273 flies. Sample number (N) per genotype: iso31Bw- N = 280, Nrx-1^273/273 N = 270, Nrx-1^241/241 N = 270. Values represent mean ± SEM. ^** = P < 0.01. ^**** = P < 0.0001.