Figure 6.

Expression levels of antioxidant-associated genes in transgenic Arabidopsis lines and WT Arabidopsis plants before and after heat stress treatments. Three-week-old transgenic Arabidopsis lines and WT Arabidopsis plants were exposed to heat stress (42°C) for 24 h and 48 h, and leaves were sampled for RNA extraction, cDNA synthesis and qPCR analysis. Gene-specific primers were used for detection of relative expression levels of stress-responsive genes, the raw date were normalized using AtActin as an internal control. For each experiment, three technical replicates were conducted. (A–P) The expression levels of AtAPX1-8 and AtGPX1-8 were determined by qRT-PCR. AtActin was used as an internal control. The error bar is calculated through three biological replications. Asterisks above the error bar indicate significant differences (*P < 0.05, **WFI 2P < 0.01) using one-way ANOVA and a Fisher’s least significant difference (LSD).