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Independent variables (Xi) and their ranges used for the zymogram optimization in the Box–Behnken design.
| Carbohydrases Factors (Xi) | Interval | Coded and Actual Levels, U/g (p = 3, γ = 1.682) 1 | ||||
|---|---|---|---|---|---|---|
| −γ | −1 | 0 | +1 | +γ | ||
| X1 EX | 9 | 39 | 45 | 54 | 63 | 69 |
| X2 EA | 1.0 | 3.3 | 4.0 | 5.0 | 6.0 | 6.7 |
| X3 EF | 0.1 | 0.23 | 0.30 | 0.40 | 0.50 | 0.57 |
1 Both the asterisk arm value (γ) and the coded level values are defined by the Design Expert Software by means of BBD design. Ternary factors (p) include EX, EA, and EF.
