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Experimental protocol of live cell imaging. Diagrams showing live cell imaging technique on (A) ex vivo specimens and (B) immortalized cell lines. Arrowheads indicate guttae. CE = corneal endothelium; DM = Descemet’s membrane; DMEK = Descemet’s membrane endothelial keratoplasty; FECD = Fuchs endothelial corneal dystrophy; HCEC = human corneal endothelial cell; LNP-GFP = lipid nanoparticle-green fluorescent protein; SV = simian virus.
