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. 2021 Mar 9;1(1):100006. doi: 10.1016/j.xops.2021.100006

Figure 2.

Figure 2

Live imaging of lipid nanoparticle (LNP)-mediated green fluorescent protein (GFP) expression in corneal endothelial cells (CECs) on normal and Fuchs endothelial corneal dystrophy (FECD) ex vivo specimens. A, Live imaging of normal and FECD ex vivo specimens. Low-magnification top panels show entire ex vivo specimens. Bottom panels show high magnification of GFP-expressing CECs on ex vivo specimens. Arrowhead indicates guttae. Scale bars = 1400 μm (top panels) and 25 μm (bottom panels). B, Paraformaldehyde-fixed ex vivo specimens showing expression of GFP in CECs of normal and FECD ex vivo specimens costained with DAPI to label DNA. Scale bars = 25 μm (bottom panels). Mag = magnification.