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. 2022 Oct 13;13:6054. doi: 10.1038/s41467-022-33547-8

Fig. 6. Human wild-type INTS13, but not mutant INTS13-F1, rescues spermatogenesis defects of Drosophila mutant ints13 males.

Fig. 6

a–j Male germline expression of wild-type, full-length human INTS13 (INTS13-wt), but not a truncated form (INTS13-F1), restored perinuclear dynein in Drosophila asun primary spermatids (a–d; quantified in e) and spermatocytes (f–i; quantified in j). Representative G2 spermatocytes and immature spermatids stained for dynein heavy chain (green) and DNA (blue) are shown. Bar graphs depict percentages of spermatids and spermatocytes showing properly localized dynein heavy chain. k–o Male germline expression of wild-type, full-length human INTS13, but not INTS13-F1, restored nucleus-centrosome coupling in asun primary spermatocytes (k–n; quantified in o). Representative prophase I spermatocytes stained for β-tubulin (green) and DNA (blue) are shown. Bar graphs depict percentages of spermatocytes showing normal nucleus-centrosome coupling. Data are mean ± s.e.m. *P < 0.0001, one-tailed Student’s t-test. e, j n > 10 flies were used with n > 584 spermatids or spermatocytes scored. o n > 10 flies were used with n > 120 spermatocytes scored. INTS13-F1 is encoded by INTS13 c.2004delA. Scale bars, 10 µm. Source data are provided as a Source Data file.