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. 2022 Sep 29;12:1017542. doi: 10.3389/fcimb.2022.1017542

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Copyright © 2022 Shola David and Kanayeva

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Specificity of the direct ELONA to SARS-CoV-2 S glycoprotein. Non-target proteins, MERS-CoV S glycoprotein, SARS-CoV-2 N protein, HA peptide, and HSA at a concentration of 500 ng/mL coupled with the CoV2-RBD-1C aptamer (1,500 nM) were employed in this study (N = 3). Nuclease-free water was used for the background control, while SARS-CoV-2 S glycoprotein coated with no aptamer served as the negative control. Absorbance was obtained by subtracting the background control from all other wells. All results are presented in ± SEMs. ****p< 0.0001.