Figure 4.

Flow cytometric analysis of myeloid lineage cells. In melanoma, (A) significant differences were seen among monocyte populations. In (B), M1 macrophages were significantly reduced in dual-treated samples compared with untreated tumors, and in (C), the M2 population was abrogated with combined therapy. In lung cancer, combined therapy led to significant increases in both monocytes, M1, and M2 macrophages compared with either treatment alone (D–F) (n = 3 per group). Significant differences are marked with a line with an asterisk. (M1 macrophages: CD80⁺/CD11b⁺; CD11B⁺/CD206⁺). Supplemental Figure S2 presents an example flow gating strategy for myeloid cell populations.