Table 2.
Formulation and proximate composition of experimental diets (% dry matter).
| Ingredients (%) | Experimental Diets | |||
|---|---|---|---|---|
| TM0 | TM15 | TM30 | TM45 | |
| Fish meal | 40.00 | 34.00 | 28.00 | 22.00 |
| Chicken meal a | 13.00 | 13.00 | 13.00 | 13.00 |
| Soybean meal | 10.00 | 10.00 | 10.00 | 10.00 |
| TM b | 0.00 | 6.70 | 13.30 | 20.00 |
| High gluten flour | 23.00 | 21.39 | 19.87 | 18.25 |
| Soybean oil | 1.50 | 1.50 | 1.50 | 1.50 |
| Fish oil | 2.50 | 2.90 | 3.30 | 3.70 |
| Lecithin | 1.50 | 1.50 | 1.50 | 1.50 |
| Stickwater c | 6.00 | 6.00 | 6.00 | 6.00 |
| Vitamin C d | 0.10 | 0.10 | 0.10 | 0.10 |
| Vitamin premix e | 0.40 | 0.40 | 0.40 | 0.40 |
| Mineral premix f | 0.50 | 0.50 | 0.50 | 0.50 |
| Ca(H2PO4)2 | 1.00 | 1.50 | 2.00 | 2.50 |
| Choline chloride | 0.50 | 0.50 | 0.50 | 0.50 |
| Methionine | 0.00 | 0.01 | 0.03 | 0.05 |
| Proximate analysis | ||||
| Dry matter | 98.02 | 98.49 | 98.59 | 98.85 |
| Crude protein | 47.98 | 48.30 | 48.00 | 48.10 |
| Crude lipid | 8.25 | 8.63 | 8.76 | 8.79 |
| Ash | 11.12 | 10.70 | 10.85 | 10.50 |
| Methionine | 2.60 | 2.64 | 2.67 | 2.71 |
| Lysine | 0.93 | 0.93 | 0.93 | 0.94 |
| Total Phosphorus | 1.38 | 1.38 | 1.38 | 1.37 |
a Chicken meal was supplied by Qingdao Bio-ways Ingredients Bio-technology Co., Ltd., Qingdao, China. b TM (Tenebrio molitor meal) was supplied by Guangzhou Z and C Biology Co., Ltd., Guangzhou, China. c Stickwater was supplied by Fujian Hongwei Bio-technology Co., Ltd., Zhangzhou, China. d The form of Vitamin C is L-Ascorbate-2-phosphate. e,f Vitamin premix and Mineral premix were prepared in our previous work [37].