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. Author manuscript; available in PMC: 2022 Oct 13.
Published in final edited form as: Neuroscience. 2020 Jul 9;442:183–192. doi: 10.1016/j.neuroscience.2020.07.006

Figure 1.

Figure 1.

Cre-Lox Expression System for Conditional Expression of C3. (1A) Design of engineered floxed adeno-associated viral (AAV-DIO-Ef1α-EYFP-P2A-C3) vectors for C3 gene delivery for long-term expression in the CNS in a cell-autonomous fashion with utilization of the Cre-Lox system for cell-specific expression. Cells expressing Cre recombinase (Cre) can invert the floxed vectors in the correct reading frame allowing for cell-specific expression. (1B) Brightfield and fluorescent micrographs demonstrating vector expression in the presence of Cre. Vectors were introduced into human embryonic kidney cells (HEK-293) with and without Cre in vitro; enhanced yellow fluorescent protein (EYFP) fluorescence was strongly visualized in cells co-transfected with Cre. Scale bar: B) 100 μm.