TABLE 1.
Comparative analysis of paratuberculous tissue sections by Ziehl-Neelsen staining and a362-based immunohistological procedures
| Type of specimen | Clinical form | Result ofa:
|
||
|---|---|---|---|---|
| ZiehlNeelsen staining | Polyclonal procedure | Monoclonal procedure | ||
| Mesenteric lymph node | Pluribacillary | + | + | + |
| Ileum | Pluribacillary | + | + | + |
| Ileum | Pluribacillary | + | + | + |
| Ileum | Pluribacillary | + | + | + |
| Ileum | Pluribacillary | + | + | + |
| Colon | Pluribacillary | + | + | + |
| Mesenteric lymph node | Paucibacillary | + | + | + |
| Mesenteric lymph node | Paucibacillary | − | + | + |
| Mesenteric lymph node | Paucibacillary | − | − | + |
| Mesenteric lymph node | Paucibacillary | − | − | − |
| Ileum | Paucibacillary | − | + | + |
| Ileum | Paucibacillary | + | + | + |
| Ileum | Paucibacillary | − | + | + |
| Ileum | Paucibacillary | − | − | + |
| Ileum | Paucibacillary | − | − | − |
| Colon | Paucibacillary | − | + | + |
a
Agreement between methods (Cohen’s K coefficient): K1 (Ziehl-Neelsen and the polyclonal procedure), 44%; K2 (Ziehl-Neelsen and the monoclonal procedure), 25%; K3 (polyclonal and monoclonal procedures), 60%. Discordance between methods (binomial test): P1 (Ziehl-Neelsen and the polyclonal procedure), 0.062; P2 (Ziehl-Neelsen and the monoclonal procedure), 0.016; P3 (polyclonal and monoclonal procedures), 0.25.