FIGURE 4.

Validation of the gene signature in different series. (A) TCGA confirmed the pyroptosis status in the validation cohort. (B) PRS was significantly decreased in patients alive in the validation cohort. (C) Patients with lower PRS exhibited better prognosis in the validation cohort. (D,E) The best k value was chosen for NMF consensus clustering in the TCGA (Figure 4D) and GEO (Figure 4E) cohorts. (F,G) Statistical difference in OS was observed in NMF-derived clusters based on the expression pattern of the gene signature (TCGA: Figure 4F; GEO: Figure 4G). (H,I) Multivariate Cox regression analysis indicated that PRS was an independent risk factor for OS in the training and validation cohorts (Figure 4H), as well as in the all cohorts (Figure 4I).