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. 2022 Oct 12;19:256. doi: 10.1186/s12974-022-02601-z

Table 2.

DAPI-uptake of microglia after icv injection of P2X7 specific nb-B mod

DAPI negative cells (in %) DAPI positive cells (in %)
no nb in vivo + no nb ex vivo + no ATP 98.6 1.4
no nb in vivo + 0.5 μg ex vivo + 0.5 mM ATP 83.3 16.7
30 μg in vivo + no nb ex vivo + 0.5 mM ATP + 160 min circulation 61.9 38.1

no nb in vivo +

no nb ex vivo + 0.5 mM ATP

10.5 89.5

Functional analysis of P2X7 activation in microglia after icv injection of nbs was tested by checking the ATP-induced DAPI-uptake (Fig. 5C)