TABLE 5.
Evaluation of addition of various antigens from heterologous strains to IgG WB of whole-cell lysates of different strains
| Basic test: WB with whole-cell lysate of straina: | Additional antigen/strain | Loss of specificity (%) | Gain of sensitivity (%)b |
|---|---|---|---|
| PKa2 | p58/PKo | 1.4 | 6.3 |
| p43/PKo | 6.4 | 8.5 | |
| p39/PKo | 1.4 | 5.3 | |
| p17/PKo | 3.5 | 6.3 | |
| p14/PKo | 0.7 | 3.2 | |
| p39/PBi | 0.0 | 3.2 | |
| OspC/PBi | 2.1 | 4.2 | |
| PKo | OspC/PBi | 2.1c | 6.9c |
| OspC/PKa2 | 2.8c | 2.6c | |
| PBi | p58/PKo | 2.8 | 6.9 |
| p43/PKo | 5.7 | 7.4 | |
| p17/PKo | 3.5 | 7.9 | |
| p14/PKo | 0.7 | 4.8 |
b
Only additional antigens leading to a gain of sensitivity of at least 3% are included.
c
Results for criterion of a reactive OspC of strain PBi or at least two reactive bands among p83/100, p58, p43, p39, p30, OspC, p21, p17, and p14 of strain PKo for a positive WB.