Figure 5. Optimized prototrophic cells double every 3.2 days in unconditioned valine-free RPMI medium over 39 days.

Population doubling level of pCtrl, pMTIV, pMTIV-ilvD + cells cultured in unconditioned, reduced-isoleucine, valine-free RPMI medium containing 2 mM sodium pyruvate (Figure 5—source data 1). Plates were not coated with gelatin. Error bars represent data from three replicates.

Figure 5—figure supplement 1. Comparing growth of cell lines with elevated ilvD copy numbers to those without.

(A) Comparison of growth in unconditioned reduced-isoleucine valine-free RPMI containing 2 mM sodium pyruvate (Figure 5—figure supplement 1—source data 1). Error bars represent data from three replicates. (B) Comparison of pMTIV and pMTIV-ilvD + long term growth in reconstituted complete RPMI (Figure 5—figure supplement 1—source data 1). Error bars represent data from three replicates.