Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (E. coli) | TransforMax EPI300 | Lucigen | C300C105 | Chemically competent |
| Strain, strain background (E. coli) | NEB 10-beta | New England Biolabs | C3020K | Electrocompetent |
| Cell line (C. griseus) | CHO Flp-In | ThermoFisher | R75807 | |
| Cell line (C. griseus) | CHO pMTIV | This paper | Cell line maintained in J.D.Boeke lab | |
| Cell line (C. griseus) | CHO pCtrl | This paper | Cell line maintained in J.D.Boeke lab | |
| Cell line (C. griseus) | CHO pMTIV-ilvD+ | This paper | Cell line maintained in J.D.Boeke lab | |
| Cell line (C. griseus) | CHO pCtrl-ilvD+ | This paper | Cell line maintained in J.D.Boeke lab | |
| Cell line (C. griseus) | CHO pPro | This paper | Cell line maintained in H.H.Wang lab | |
| Cell line (C. griseus) | CHO pCtrl-mCh | This paper | Cell line maintained in H.H.Wang lab | |
| Recombinant DNA reagent | pJTR0381 (plasmid) | This paper | Encodes Ec ilvD transcription units for lentiviral transduction | |
| Antibody | Anti-2A peptide (mouse monoclonal) | Novus Biologicals | NBP2-59627 | 1:1000 dilution |
| Antibody | Anti-α/β-tubulin (rabbit polyclonal) | Cell Signaling Technology | 2148 | 1:1000 dilution |
| Antibody | IRDye 800CW Anti-mouse IgG (goat polyclonal) | LI-COR | 926–32210 | 1:20000 dilution |
| Antibody | IRDye 680 RD Anti-rabbit IgG (goat polyclonal) | LI-COR | 926–68071 | 1:20000 dilution |
| Commercial assay or kit | PrestoBlue Cell Viability Reagent | ThermoFisher | A13261 | |
| Commercial assay or kit | NEBNext poly(A) mRNA Magnetic Isolation module | New England Biolabs | E7490 | |
| Commercial assay or kit | NEBNext Ultra RNA Library Prep Kit for Illumina | New England Biolabs | E7770 | |
| Other | F-12K medium without amino acids, powder base | US Biological | N8545 | Dropout medium base |
| Other | RPMI 1640 without amino acids & glucose, powder base | US Biological | R9010-01 | Dropout medium base |
| Other | Gibco fetal bovine serum, dialyzed | Fisher Scientific | 26400044 | Lot 2140178 |
CHO: Chinese hamster ovary.