Video 1. Collagen-rich region at branchpoint.

Download video file ^{(12.2MB, mp4)}

The video is a 3D rendering of the image depicted in Figure 1B, showing branching airways and blood vessels (BV) in the lung of a naïve Shh-Cre ROSA26-mTmG mouse. The image was stitched from tiled images collected by two-photon microscopy of a precision-cut thick vibratome section. Bronchial airway and alveolar epithelial cells express membrane-bound GFP (green), whereas all other cells express tdTomato (red). Collagen (blue) was visualized by second harmonic generation.