Video 3. CD11c-YFPbright DCs are positioned near the bronchial airway epithelium and are enriched at collagen-rich regions near branchpoints.
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The video is a 3D rendering of the image depicted in Figure 1H (right panel), showing branching airways in the lung of a naïve mouse expressing CD11c-YFP (green) to visualize DCs, together with Shh-Cre and the Ai14 (tdTomato) reporter to show airway and alveolar epithelial cells (blue). Collagen (magenta) was visualized by second harmonic generation. The image was stitched from tiled images collected by two-photon microscopy of a precision-cut thick vibratome section.