Video 4. Antigen-capturing BAMs interact with DCs.

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The video is a time-lapse image sequence showing the interactions of CX3CR1-GFP^bright BAMs (green) with CD11c-YFP^bright DCs (yellow). The BAMs appear to have captured 10 kDa TRITC-dextran (red), which was administered to the mouse by intranasal droplet approximately 2 hr prior to lung isolation. The image sequence is rendered from a 32 μm z-stack collected by two-photon microscopy of a cultured precision-cut vibratome section of the lung. Elapsed time is shown as hh:mm:ss.