Appendix 1—figure 1. A spatial period gradient emerges in the tissue even upon entrainment of the segmentation clock.

Left: Schematic of pipeline to generate period and phase movies using pixel-by-pixel wavelet analysis (A). Illustration by Stefano Vianello. Right: Snapshot of period wavelet movie of control subjected to 170 min periodic pulses of DMSO (B) or entrained sample subjected to 170 min periodic pulses of 2 µM DAPT (C), taken at 840 min after the start of experiment. Period is either shown using heatmap (B1,C1) or as a surface plot (B2,C2). Bottom: Snapshots of period wavelet movie of samples in (B) and (C) at different time points. Time is indicated as minutes elapsed from the start of the experiment. Sample is rotated so that the dorsal side is up. Timelapse of period wavelet movies and corresponding surface plots are available at https://youtu.be/3Y53OhXacKI.

Appendix 1—figure 1—video 1. Period gradient of LuVeLu in E10.5 2D-assay subjected to 170 min periodic pulses of 2 µM DAPT (or DMSO for control) available at https://youtu.be/3Y53OhXacKI.

Download video file ^{(8.9MB, mp4)}

Appendix 1—figure 1—video 2. E10.5 2D-assay, expressing LuVeLu, subjected to 170 min periodic pulses of DMSO control, with corresponding period and phase wavelet movies, available at https://github.com/PGLSanchez/EMBL-files/blob/master/MOVIES/SO_2.0D_170mins_CTRL.avi.

Download video file ^{(4.9MB, mp4)}

Appendix 1—figure 1—video 3. E10.5 2D-assay, expressing LuVeLu, subjected to 170 min periodic pulses of 2 µM DAPT, with corresponding period and phase wavelet movies, available at https://github.com/PGLSanchez/EMBL-files/blob/master/MOVIES/SO_2.0D_170mins_DAPT.avi.

Download video file ^{(4.4MB, mp4)}

Appendix 1—figure 1—video 4. E10.5 2D-assay, expressing LuVeLu, subjected to 130 min periodic pulses of DMSO control, with corresponding period and phase wavelet movies, available at https://github.com/PGLSanchez/EMBL-files/blob/master/MOVIES/SO_2.0D_130mins_CTRL.avi.

Download video file ^{(4.6MB, mp4)}

Appendix 1—figure 1—video 5. E10.5 2D-assay, expressing LuVeLu, subjected to 130 min periodic pulses of 2 µM DAPT, with corresponding period and phase wavelet movies, available at https://github.com/PGLSanchez/EMBL-files/blob/master/MOVIES/SO_2.0D_130mins_DAPT.avi.

Download video file ^{(6.1MB, mp4)}