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. 2022 Sep 29;13:926696. doi: 10.3389/fimmu.2022.926696

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Copyright © 2022 Tomer, Mu, Suryawanshi, Ng, Wang, Wennerberg, Rezek, Martin, Chen, Kitchen and Zhen

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Cannabidiol treatment leads to increased autophagy activity. (A) Real time PCR analysis of p62 expression in THP-1 cells treated with either vehicle or CBD for 8 hours. (B) THP1 cells were treated with CBD overnight, fixed and stained with DAPI, anti-p62 and analyzed by ImageStream. (C) Bar graph showing mean Spot count of p62 for mock and CBD treated THP1 analyzed by ImageStream IDEA software. (D) Western blot of THP1 cells treated with DMSO, CBD, cGAMP or CBD+cGAMP overnight. (E) Bar graph showing LCI/LCII ratio and p62/actin of the western blot analyzed by Image Lab Software. ****p<0.0001.