Figure 5.

CBD suppresses ISG MX1 expression level and increase HIV RNA level 24 hours after infection in THP-1 cells and macrophages. THP-1 cells were stimulated for 5 days with PMA and primary monocytes were differentiated using M-CSF for 5 days. Cells were treated with vehicle or CBD for 24 hours followed by infection with HIV for 24 hours and harvested. Fold change of expression level of MX1/HPRT1 compared to mock in (A) THP-1 cells and (B) CD14+ primary macrophages. Fold change of HIV RNA expression level compared to vehicle treated HIV infected (C) THP-1 cells or (D) CD14+ primary macrophages as measured by real time PCR. *p<0.05, **p<0.005, ***p<0.0005.