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. 2022 Sep 29;13:1020159. doi: 10.3389/fimmu.2022.1020159

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Copyright © 2022 Rudi, Martin Aispuro, Zurita, Gonzalez Lopez Ledesma, Bottero, Malito, Gabrielli, Gaillard, Stuible, Durocher, Gamarnik, Wigdorovitz and Hozbor

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Neutralizing capacity against W or D pseudovirus induced by a booster with SW-Vac 2µg formulation. BALB/c mice were immunized on Days 0 and 14 with commercial Gam-COVID-Vac-rAd26/rAd5 or AZD1222/ChAdOx1 vaccine plus a booster with SW-Vac formulation, Gam-COVID-Vac-rAd5 or AZD1222/ChAdOx1 on Day 49 delivered intramuscularly (A). Serum was collected on Day 14 after the last dose. Neutralizing titers were measured by 50% inhibition for the pseudotyped virus (CoV2pp-GFP) in immunized and non-immunized mice (B). Absolute inhibitory concentration was calculated as the corresponding point between the 0% and 100% assay controls. Fifty % inhibition was defined by the controls for all the samples on the same plate. Data is presented as the fold change of CoV2pp-GFP related to the minimum value detected in immunized animals. For statistical analysis, one-way ANOVA with Bonferroni`s multiple comparisons test was used. ****p < 0.0001.