Key resources table
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Chicken polyclonal anti-GFP | AVES |
Cat # GFP-1020;
RRID: AB_10000240 |
| Goat polyclonal anti-choline acetyltransferase | Millipore |
Cat # AB144P;
RRID: AB_11214092 |
| Rabbit polyclonal anti-DsRed | Living Colors |
Cat # 632496;
RRID: AB_10013483 |
| Guinea pig polyclonal anti-RBPMS | PhosphoSolutions |
Cat# 1832-RBPMS;
RRID: AB_2492226 |
| Rabbit polyclonal anti-calbindin D-28k | Swant |
Cat # CB38;
RRID: AB_10000340 |
| Brilliant Violet 421 rat anti-CD11b | BioLegend |
Cat # 101251;
RRID:AB_2562904 |
| Bacterial and virus strains | ||
| AAV9 FLEX-tdTomato (6.8E+12) | UNC Vector Core | N/A |
| AAV9 FLEX-GFP (3.7E+12) | UNC Vector Core | N/A |
| AAV2 FLEX-GFP (3.7E+12) | UNC Vector Core | N/A |
| Biological samples | ||
| Chemicals, peptides, and recombinant proteins | ||
| Papain | Worthington | Cat # LS003126 |
| DAPI (4’, 6-Diamidino-2-Phenylindole, Dihydrochloride) | Life Technologies |
Cat # D1306;
RRID: AB_2629482 |
| Cholera Toxin Subunit B (Recombinant), Alexa Fluor 555 Conjugate | Thermo Fisher | Cat # C34776 |
| Cholera Toxin Subunit B (Recombinant), Alexa Fluor 647 Conjugate | Thermo Fisher | Cat # C34778 |
| Propidium iodide | Thermo Fisher | Cat # P1304MP |
| Critical commercial assays | ||
| Smart-seq2 reagents | Picelli et al. 50 | N/A |
| Nextera XT DNA Library Preparation Kit | Illumina | Cat # FC-131-1024 |
| RNAscope Fluorescent Multiplex Kit | Advanced Cell Diagnostics | Cat # 320850 |
| RNAscope Probe – Mm-Sfrp1 | Advanced Cell Diagnostics | Cat # 404981 |
| RNAscope Probe – eGFP | Advanced Cell Diagnostics | Cat # 400281 |
| RNAscope Probe – GFP-C2 | Advanced Cell Diagnostics | Cat # 409011-C2 |
| RNAscope Probe – Mm-Tbx5-C2 | Advanced Cell Diagnostics | Cat # 519581-C2 |
| RNAscope Probe – Mm-Chat-C3 | Advanced Cell Diagnostics | Cat # 410071-C3 |
| RNAscope Probe – Mm-Fibcd1-C3 | Advanced Cell Diagnostics | Cat # 524021-C3 |
| RNAscope 3-plex Negative Control Probe | Advanced Cell Diagnostics | Cat # 320871 |
| RNAscope 3-plex Positive Control Probe Mm | Advanced Cell Diagnostics | Cat # 320881 |
| Reagent or resource | ||
| Superscript III First-Strand Synthesis SuperMix | Thermo Fisher | Cat # 18080400 |
| Deposited data | ||
| Raw data files for scRNA-sequencing | This study | |
| Experimental models: Cell lines | ||
| Experimental models: Organisms/strains | ||
| Mouse: Spig1-GFP | Yonehara et al. 19 | N/A |
| Mouse: Tg(Pcdh9-cre)NP276Gsat/Mmucd | MMRRC |
MMRRC Stock # 036084-UCD;
RRID: MMRRC_036084-UCD |
| Mouse: Tbx5 flox/+ |
Gift from B.
Bruneau |
N/A |
| Mouse: B6.Cg-Npy tm1(cre)Zman /J | Jackson Laboratory |
Jackson Laboratory Stock # 027851;
RRID: IMSR_JAX:027851 |
| Mouse: B6.Cg-Gt(ROSA)26Sor tm14(CAG-tdTomato)Hze /J | Jackson Laboratory |
Jackson Laboratory Stock # 007914;
RRID: IMSR_JAX:007914 |
| Mouse: Sfrp1 +/− | Gift from J. Nathans | N/A |
| Oligonucleotides | ||
| Recombinant DNA | ||
| Software and algorithms | ||
| Tophat | Trapnell et al. 53 | RRID: SCR_013035 |
| Cufflinks | Trapnell et al. 53 |
http://cole-traonell-lab.github.io/cufflinks/; RRID: SCR_014597 |
| Cuffdiff | Trapnell et al. 53 |
http://cole-traonell-lab.github.io/cufflinks/; RRID: SCR_001647 |
| R version 3.5.1 | The R project | https://www.r-project.org/ |
| ImageJ | NIH |
https://imagej.nih.gov/ij/; RRID: SCR_003070 |
| Igor Pro version 6.37 | WaveMetrics | RRID: SCR_000325 |
| MATLAB_R2012a version 7.14.0.739 | MathWorks | RRID: SCR_001622 |
| LabVIEW 2012 version 12.0f3 |
National
Instruments |
RRID: SCR_014325 |
| Other | ||
| Illumina Nextseq 500 | Illumina | N/A |