| Antibodies |
| Rabbit monoclonal anti-cleaved PARP |
Cell signaling technology |
Cat# 5625S RRID: AB_10699459 |
| Mouse monoclonal anti-β-actin |
Cell signaling technology |
Cat# 3700S RRID: AB_2242334 |
| Rabbit monoclonal anti-LKB1 |
Cell signaling technology |
Cat# 3047S RRID: AB_2198327 |
| Rabbit monoclonal anti-cGAS |
Cell signaling technology |
Cat#15102S RRID: AB_2732795 |
| Rabbit monoclonal anti-STING |
Cell signaling technology |
Cat#13647S AB 732796 |
| Rabbit monoclonal anti-STING (Rodent preferred) |
Cell signaling technology |
Cat#50494S RRID: AB_2799375 |
| Rabbit monoclonal anti-phospho STAT1 (Tyr701) |
Cell signaling technology |
Cat#9167S RRID: AB_561284 |
| Rabbit polyclonal anti-STAT1 |
Cell signaling technology |
Cat#9172S RRID: AB_2198300 |
| Rabbit polyclonal anti-IFNAR1 |
Thermo Fisher Scientific |
Cat#A304-290A RRID: AB_2620486 |
| Rabbit monoclonal anti-phospho-Histone H2A.X (Ser139) |
Cell signaling technology |
Cat#9718S RRID: AB_2118009 |
| Rabbit monoclonal anti-Histone H3 |
Cell signaling technology |
Cat#4499S RRID: AB_10544537 |
| Rabbit monoclonal anti-phospho-TBK1/NAK (Ser172) |
Cell signaling technology |
Cat#5483S RRID: AB_10693472 |
| Rabbit monoclonal anti-TBK1/NAK |
Cell signaling technology |
Cat#3013S RRID: AB_2199749 |
| Rabbit monoclonal anti-DNMT1 |
Cell signaling technology |
Cat#5032S RRID: AB_10548197 |
| Mouse monoclonal anti- PCNA |
Dako |
Cat#M0879 RRID: AB_2160651 |
| Rabbit monoclonal anti-Aurora B/AIM1 |
Cell signaling technology |
Cat# 3094 RRID: AB_10695307 |
| Mouse monoclonal anti-TTK/MPS1 |
abcam |
Cat# ab11108 RRID: AB_297757 |
| Rabbit monoclonal anti-Lamin B2 |
abcam |
Cat#ab151735 RRID: AB_2827514 |
| Alexa Fluor 700 anti-mouse CD3 |
Biolegend |
Cat#100216 RRID: AB_493697 |
| Brilliant Violet 711 anti-mouse CD4 |
Biolegend |
Cat# 100550 RRID: AB_2562099 |
| PE/Dazzle 594 anti-mouse CD8a |
Biolegend |
Cat# 100762 RRID: AB_2564026 |
| Brilliant Violet 711 anti-mouse/human CD11b |
Biolegend |
Cat# 101242 RRID: AB_11218791 |
| Brilliant Violet 605 anti-mouse CD19 |
Biolegend |
Cat# 115540 RRID: AB_11203538 |
| Brilliant Violet 785 anti-mouse CD25 |
Biolegend |
Cat# 102051 RRID: AB_2564131 |
| Brilliant Violet 510™ anti-mouse CD45 |
Biolegend |
Cat# 103138 RRID: AB_2561392 |
| PerCP/Cyanine5.5 anti-mouse CD49b |
Biolegend |
Cat# 108916 RRID: AB_2129358 |
| PerCP/Cyanine5.5 anti-mouse Ly-6G |
Biolegend |
Cat# 127616 RRID: AB_1877271 |
| PE anti-mouse CD223 (LAG-3) |
Biolegend |
Cat# 125208 RRID: AB_2133343 |
| Brilliant Violet 421 anti-mouse CD279 (PD-1) |
Biolegend |
Cat# 135218 RRID: AB_2561447 |
| APC anti-mouse CD366 (Tim-3) Antibody |
Biolegend |
Cat# 134008 RRID: AB_2562998 |
| FOXP3 Monoclonal Antibody (FJK-16s), PE-Cyanine7 |
Thermo Fisher Scientific |
Cat# 25-5773-82 RRID: AB_891552 |
| FITC anti-human CD183 (CXCR3) |
Biolegend |
Cat# 353703 RRID: AB_10962910 |
| FITC anti-human HLA-A,B,C |
Biolegend |
Cat# 311404 RRID: AB_314873 |
| PE/Cyanine7 anti-human CD274 (B7-H1, PD-L1) |
Biolegend |
Cat# 329738 RRID: AB_2617010 |
| PerCP/Cyanine5.5 Mouse IgG2b, κ Isotype Ctrl |
Biolegend |
Cat# 400338 RRID: AB_10765682 |
| FITC Mouse IgG2a, κ Isotype Ctrl |
Biolegend |
Cat# 400208 RRID: AB_10764135 |
| STING Polyclonal Antibody |
Thermo Fisher Scientific |
Cat# PA5-23381 RRID: AB_2540904 |
| ERGIC53 Monoclonal monoclonal antibody |
AXXORA |
Cat# PSC-PM-7213-C100 |
| CD3ε (D4V8L) Rabbit mAb |
Cell signaling technology |
Cat# 99940 RRID: AB_2755035 |
| CD8α (D4W2Z) XP Rabbit mAb (Mouse Specific) |
Cell signaling technology |
Cat# 98941 AB 2756376 |
| InVivoMAb anti-mouse CD8α |
Bio X cell |
Cat# BE0004-1 RRID: AB_1107671 |
| Biological Samples |
|
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| Patient-derived cell lines |
Köhler et al. 2021
|
N/A |
| PBMC |
STEMCELL |
Cat#70025 |
|
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| Chemicals, Peptides, and Recombinant Proteins |
|
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| 2’3’-cGAMP |
Invivogene |
Cat# tlrl-nacga23 CAS#:1441190-66-4 |
| ADU-S100 |
Chemietek |
Cat# CT-ADUS100 CAS#:1638750-95-4 |
| ruxolitinib |
Selleck chemicals |
Cat# S1378 CAS#:941678-49-5 |
| cisplatin |
Sigma Aldrich |
Cat# 232120 CAS#: 15663-27-1 |
| docetaxel |
Selleck chemicals |
Cat# S1148 CAS#:114977-28-5 |
| etoposide |
Sigma Aldrich |
Cat# 341205 CAS#:33419-42-0 |
| vinorelbine |
Sigma Aldrich |
Cat# V2264 CAS#:125317-39-7 |
| pemetrexed |
Selleck chemicals |
Cat# S1135 CAS#:150399-23-8 |
| methotrexate |
Sigma Aldrich |
Cat# A6770 CAS#:133073-73-1 |
| aminopterin |
Sigma Aldrich |
Cat# A1784 CAS#:54-62-6 |
| nocodazole |
Sigma Aldrich |
Cat# M1404 CAS#:31430-18-9 |
| hydroxyurea |
Sigma Aldrich |
Cat# H8627 CAS#:127-07-1 |
| olaparib |
Selleck chemicals |
Cat# S1060 CAS#:763113-22-0 |
| barasertib |
Cayman Chemical |
Cat# 11602 CAS#:722544-51-6 |
| MK5108 |
Cayman Chemical |
Cat# 21266 CAS#:1010085-13-8 |
| ceralasertib |
Cayman Chemical |
Cat# 21035 CAS#:1352226-88-0 |
| prexasertib |
Cayman Chemical |
Cat# 21490 CAS#:1234015-52-1 |
| CFI-402257 |
Cayman Chemical |
Cat# 21960 CAS#:1610759-22-2 |
| volasertib |
Cayman Chemical |
Cat# 18193 CAS#:755038-65-4 |
| BAY-1217389 |
Selleck chemicals |
Cat# S8215 CAS#:1554458-53-5 |
| CC-671 |
MedChemExpress |
Cat# HY-108709 CAS :1618658-88-0 |
| Human IFN-γ |
R&D systems |
Cat# 285-IF-100 |
| Human IL-2 |
Thermo Fisher Scientific |
Cat# PHC0021 |
| Human IL-7 |
Thermo Fisher Scientific |
Cat# PHC0075 |
| Human IL-15 |
Thermo Fisher Scientific |
Cat# PHC9154 |
| decitabine |
Selleck chemicals |
Cat# S1200 CAS#:2353-33-5 |
| GSK126 |
Selleck chemicals |
Cat# S7061 CAS#:1346574-57-9 |
| Poly(dA:dT) |
InvivoGen |
Cat# tlrl-patn CAS#:86828-69-5 |
| Critical Commercial Assays |
|
|
| Human IFN-β ELISA kit |
Thermo Fisher Scientific |
Cat# 414101 |
| HumanCXCL10 ELISA kit |
R&D systems |
Cat# DIP100 |
| Human 2’3’-cGAMPv ELISA kit |
Cayman Chemical |
Cat# 501700 |
| Human Granzyme B ELISA kit |
R&D systems |
Cat# DGZB00 |
| Mouse Cytokine/Chemokine Magnetic Bead Panel |
Merck Millipore |
Cat# MCYTMAG-70K-PX32 |
| CellTiter-Glo luminescent cell viability assay kit |
Promega |
Cat# G7572 |
| Deposited Data |
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| Experimental Models: Cell Lines |
|
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| Human: A549 |
ATCC |
CCL-185 |
| Human: HCC44 |
Broad Institute |
N/A |
| Human: NCI-H23 |
ATCC |
CRL-5800 |
| Human: NCI-H1355 |
ATCC |
CRL-5865 |
| Human: NCI-H2122 |
ATCC |
CRL-5985 |
| Human: NCI-H2030 |
ATCC |
CRL-5914 |
| Human: NCI-H2009 |
ATCC |
CRL-5911 |
| Human: NCI-H1792 |
ATCC |
CRL-5895 |
| Human: NCI-H441 |
ATCC |
HTB-174 |
| Human: NCI-H358 |
ATCC |
CRL-5807 |
| Human: NCI-H1944 |
ATCC |
CRL-5907 |
| Human: NCI-H647 |
ATCC |
CRL-5834 |
| Human: A-427 |
ATCC |
HTB-53 |
| Human: Jurkat, Clone E6-1 |
ATCC |
TIB-152 |
| Human: HEK293T |
ATCC |
CRL-3216 |
| Human: NK-92 |
ATCC |
CRL-2407 |
| Human: THP-1 |
ATCC |
TIB-202 |
| Human: DFCI-24 |
Köhler et. al. 2021
|
N/A |
| Human: DFCI-298 |
Köhler et. al. 2021
|
N/A |
| Human: DFCI-316 |
Köhler et. al. 2021
|
N/A |
| Human: DFCI-332 |
Köhler et. al. 2021
|
N/A |
| Mouse: LLC |
ATCC |
CRL-1642 |
| Mouse: CMT167 |
ECACC |
10032302 |
| Mouse: 393P |
Gibbons et. al. 2009 |
N/A |
| Mouse: Lacun3 |
Bleau et. al. 2014 |
N/A |
| Experimental Models: Organisms/Strains |
|
|
| Mouse: 129S2/SvPasCrl |
Charles River Laboratories |
Strain #: 476 |
| Mouse: NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ |
The Jackson Laboratory |
Strain #: 005557 |
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| Recombinant DNA |
|
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| pLX304-NanoLuc |
Kitajima et al 2019
|
N/A |
| pLX304-human LKB1 |
Kitajima et al 2019
|
N/A |
| pLX304-human STING |
Kitajima et al 2019
|
|
| pLX304-human cGAS |
Campisi et al 2020
|
|
| pLX307-human CXCR3 |
Kitajima et al 2019
|
|
| pCRISPRv2-puro-scramble sgRNA control, see Table S2
|
Broad Institute |
N/A |
| pCRISPRv2-puro-human LKB1 sgRNA, see Table S2
|
Kitajima et al 2019
|
|
| pCRISPRv2-puro-human cGAS sgRNA, see Table S2
|
Campisi et al 2020
|
|
| pCRISPRv2-puro-human STING sgRNA, see Table S2
|
Campisi et al 2020
|
|
| pCRISPRv2-puro-human IFNAR1 sgRNA, see Table S2
|
This paper |
|
| pCRISPRv2-puro-human STAT1 sgRNA, see Table S2
|
Campisi et al 2020
|
|
| pCRISPRv2-puro-human ATG5 sgRNA, see Table S2
|
This paper |
|
| pCRISPRv2-puro-mouse scramble sgRNA control, see Table S2
|
This paper |
|
| pCRISPRv2-puro-mouse LKB1 sgRNA, see Table S2
|
This paper |
|
| pCRISPRv2-blast-mouse STING sgRNA, see Table S2
|
This paper |
|
| pCMV-dR8.91 (lentivirus packaging) |
Broad Institute |
N/A |
| pCMV-VSV-G |
Broad Institute |
N/A |
| Sequence-Based Reagents |
|
N/A |
| Primers for qRT-PCR, see Table S2
|
This paper |
|
| Software and Algorithms |
|
|
| GraphPad Prism7 |
GraphPad Software, Inc. |
http://www.graphpad.com/
|
| sgRNA designer |
Broad Institute |
http://portals.broadinstitute.org/gpp/public/analysis-tools/sgrna-design
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| Other |
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