Key Resources Table
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Anti-mouse CD117 (c-kit) BV421 | Biolegend | Cat# 105827; RRID: AB_10898120 |
| Anti-mouse CD117 (c-kit) Pe/Dazzle 594 | Biolegend | Cat #105834; RRID: AB_ 2564055 |
| Anti-mouse CD115 (CSF-1R) PE | Biolegend | Cat# 135505; RRID: AB_1937254 |
| Anti-mouse IFNAR-1 APC | Biolegend | Cat# 127313; RRID: AB_2122746 |
| Anti-mouse Ly-6A/E (Sca-1) BV785 | Biolegend | Cat# 108139; RRID: AB_2565957 |
| Anti-mouse CD45 AF700 | Biolegend | Cat# 103128; RRID: AB_493715 |
| Anti-mouse CD45 FITC | Invitrogen | Cat# 11-0451-85; RRID: AB_465050 |
| Anti-mouse CD45 BV421 | Biolegend | Cat# 103133; RRID: AB_10899570 |
| Anti-mouse CD45 BV785 | Biolegend | Cat# 103149; RRID: AB_2564590 |
| Anti-mouse CD45 APC | Biolegend | Cat# 103112; RRID: AB_312977 |
| Anti-mouse I-A/I-E (MHCII) BV421 | Biolegend | Cat# 107632; RRID: AB_26508 |
| Anti-mouse I-A/I-E (MHCII) FITC | Invitrogen | Cat# 11-5321-82; RRID: AB_465232 |
| Anti-mouse MHC Class II (I-A/I-E) PE | Tonbo | Cat# 50-5321-U100; RRID: AB_2621796 |
| Anti-mouse H-2kb PE | BD Biosciences | Cat# 553570; RRID: AB_394928 |
| Anti-mouse H-2Db (KH95) FITC | BD Biosciences | Cat# 553573; RRID: AB_394931 |
| Anti-mouse CD80 PE | eBioscience | Cat# 12-0801-82; RRID: AB_465752 |
| Anti-mouse CD40 PE | BD Biosciences | Cat# 553791; RRID: AB_395055 |
| Anti-mouse CD86 PE | eBiosceince | Cat# 12-0862-82; RRID: AB_465768 |
| Anti-mouse CD86 APC | Biolegend | Cat# 105011; AB_493343 |
| Anti-mouse Ly-6C BV785 | Biolegend | Cat# 12804; RRID: AB_2565852 |
| Anti-mouse Ly-6C PE | BD Biosciences | Cat# 560592; RRID: AB_1727556 |
| Anti-mouse Ly-6C APC | BD Biosciences | Cat# 560595; RRID: AB_1727554 |
| Anti-mouse Ly-6C AF700 | Biolegend | Cat# 128023; RRID: AB_10640119 |
| Anti-mouse Ly-6C FITC | BD Biosciences | Cat# 553104; RRID: AB_394628 |
| Anti-mouse Ly-6G PE | BD Biosciences | Cat# 551461; RRID: AB_394208 |
| Anti-mouse Ly-6G Pe/Dazzle 594 | Biolegend | Cat# 127648; RRID: AB_2566319 |
| Anti-mouse Ly-6G FITC | BD Biosciences | Cat# 551460; RRID: AB_394207 |
| Anti-mouse Ly-6G AF700 | Biolegend | Cat# 127622; RRID: AB_10643269 |
| Anti-mouse Ly-6G APC | Tonbo | Cat# 20-1276-U100; RRID: AB_2621589 |
| Anti-mouse Ly-6G/Ly-6C (Gr-1) FITC | Biolegend | Cat# 108406; RRID: AB_313371 |
| Anti-mouse Ly-6G/Ly-6C (Gr-1) PE | BD Biosciences | Cat# 553128; RRRID: AB_394644 |
| Anti-mouse Ly-6G/Ly-6C (Gr-1) PE/Dazzle 594 | Biolegend | Cat# 108452; RRID: AB_2564249 |
| Anti-mouse Ly-6G/Ly-6C (Gr-1) APC | Biolegend | Cat# 108412; RRID: AB_313377 |
| Anti-mouse CD11c VioletFluor 450 | Tonbo | Cat# 75-0114-U025; RRID: AB_2621937 |
| Anti-mouse CD11c BV421 | Biolegend | Cat# 117329; RRID: AB_10897814 |
| Anti-mouse CD11c APC | Tonbo | Cat# 20-0114-U100; RRID: AB_2621557 |
| Anti-mouse CD11c PE | eBiosciences | Cat# 12-0114-82; RRID: AB_465552 |
| Anti-mouse CD11c BV785 | Biolegend | Cat# 117336; RRID: AB_2565268 |
| Anti-mouse CD11b PE/Dazzle 594 | Biolegend | Cat# 101256; RRID: AB_2563648 |
| Anti-mouse CD11b PE | BD Biosciences | Cat# 553311; RRID: AB_ 394775 |
| Anti-mouse CD11b BV421 | Biolegend | Cat# 101251; RRID: AB_2562904 |
| Anti-mouse CD11b APC | Tonbo | Cat# 20-0112-U100; RRID: AB_2621556 |
| Anti-mouse CD11b FITC | eBiosciences | Cat# 11-0112-85; RRID: AB_464936 |
| Anti-mouse CD11c biotin | Tonbo | Cat# 30-0114-U100 |
| Anti-mouse CD103 BV421 | Biolegend | Cat# 121421; RRID: AB_10900074 |
| Anti-mouse CD103 APC | Biolegend | Cat# 121414; RRID: AB_1227502 |
| Anti-mouse CCR2 PE | Biolegend | Cat# 150609; RRID: AB_2616981 |
| Anti-CD4 FITC | Tonbo | Cat# 35-0042-U500; RRID: AB_2621666 |
| Anti-CD4 APC | Tonbo | Cat# 20-0042-U100; RRID: AB_2621544 |
| Anti-CD4 AF700 | Biolegend | Cat# 100430; RRID: AB_493699 |
| Anti-CD4 PE | BD Biosciences | Cat# 553652; RRID: AB_394972 |
| Anti-CD8a FITC | BD Biosciences | Cat# 553031; RRID: AB_394569 |
| Anti-CD8a BV785 | Biolegend | Cat# 100749; RRID: AB_11218801 |
| Anti-CD8a PE | BD Biosciences | Cat# 553033; RRID: AB_394571 |
| Anti-CD8a BV421 | Biolegend | Cat# 100753; RRID: AB_2562558 |
| Anti-CD8a APC | Biolegend | Cat# 100712; RRID: AB_312751 |
| Anti-CD44 Pe/dazzle 594 | Biolegend | Cat# 103055; RRID: AB_2564043 |
| Anti-CD44 BV785 | Biolegend | Cat# 103041; RRID: AB_11218802 |
| Anti-CD44 FITC | Biolegend | Cat# 103022; RRID: AB_493685 |
| Anti-CD44 APC | Invitrogen | Cat# 17-0441-82; RRID: AB_469390 |
| Anti-CD69 PE | BD Biosciences | Cat# 553237; RRID: AB_394726 |
| Anti-CD69 PE/dazzle 594 | Biolegend | Cat# 104536; RRID: AB_2565583 |
| Anti-CD274 APC | Biolegend | Cat# 124311; RRID: AB_10612935 |
| Anti-CD274 BV421 | Biolegend | Cat# 124315; RRID: AB_10897097 |
| Anti-TNFα APC | Biolegend | Cat# 506307; RRID: AB_315428 |
| Anti-TNFα PE | Biolegend | Cat# 506306; RRID: AB_315427 |
| Anti-IFNγ APC | Biolegend | Cat# 505810; RRID: AB_315404 |
| Anti-IFNγ PE | BD Biosciences | Cat# 554412; RRID: AB_395376 |
| Anti-IFNγ BV421 | Biolegend | Cat# 505829; RRID: AB_10897937 |
| Anti-Phospho STAT1 (Ser727), Recombinant rabbitt monoclonal APC | Invitrogen | Cat# MA5-28056; RRID: AB_2745055 |
| Anti-Mouse Calreticulin (1G6A7) APC | Novus | Cat# NBP1-47518APC; RRID: AB_10010469 |
| Purified rat anti-mouse CD16/32 | BD Biosciences | Cat# 553142; RRID: AB_394657 |
| Anti-mouse Ki67 APC | Biolegend | Cat# 652406; RRID: AB_2561930 |
| Anti-mouse Ki67 PE | Biolegend | Cat# 652403; RRID: AB_2561524 |
| Anti-SEC61b APC | LSBio | Cat# LS-C268919 |
| Anti-p84 | Abcam | Cat #ab487; RRID: AB_304696 |
| Anti-PERK (C33E10) Rb mAb | Cell Signaling | Cat# 3192; RRID: AB_2095847 |
| Anti-Phospho-PERK (Thr980) Rb mAb | Cell Signaling | Cat# 3179; RRID: AB_2095853 |
| Anti-IRE1α (14C10) Eb mAb | Cell Signaling | Cat# 3294; RRID: AB_823545 |
| Anti-Phospho-IRE1α (S274) Rb polyAb | Abcam | Cat# 48187; RRID: AB_873899 |
| Anti-Chop Rb polyAb | SantaCruz | Cat# Sc-793; RRID: AB_631364 |
| Anti-Caspase-3 (8G10) Rabbit mAb | Cell Signaling | Cat# 9665; RRID: AB_2069872 |
| Anti-eIf2α antibody Rabbit polyclonal | Cell Signaling | Cat# 9722; RRID: AB_2230924 |
| Anti-Phospho-eIf2α Rabbit | Cell Signaling | Cat# 9721; RRID: AB_330951 |
| Anti-XBP-1s (E9V3E) | Cell Signaling | Cat# 40435; RRID: AB_2891025 |
| Anti-BiP (C50B12) | Cell Signaling | Cat# 3177; RRID: AB_2119845 |
| Anti-B-actin mAb | Sigma | Cat# A2228-100ul; RRID: AB_476697 |
| Anti-Vinculin mAb | Sigma | Cat# V9131-2ML; RRID: AB_477629 |
| Anti-ATF4 | Cell signaling | Cat# 11815; RRID: AB_2616025 |
| Anti-CD31 | Abcam | Cat# ab28364; RRID: AB_726362 |
| InVivoMAb Anti-mouse CD8a | BioXCell | Cat# BE0004-1; RRID: AB_1107671 |
| InVivoMAb Anti-mouse CD4 | BioXCell | Cat# BE0003-1; RRID: AB_1107636 |
| InVivoMAb Anti-mouse IFNAR-1 | BioXCell | Cat# BE0241; RRID: AB_2689923 |
| InVivoMAb polyclonal rat IgG | BioXCell | Cat# BE0094; RRID: AB_1107795 |
| InVivoPlus Anti-mouse PD-1 (CD279) | BioXCell | Cat# BP0146; RRID: AB_10949053 |
| InVivoMAb mouse IgG1 isotype control | BioXCell | Cat# BE0083; RRID: AB_1107784 |
| InVivoMAb polyclonal human IgG | BioXCell | Cat# BE0092; RRID: AB_1107779 |
| Ultra-LEAF Purified Anti-HMGB1 blocking antibody | Biolegend | Cat# 651413; RRID: AB_2728487 |
| IFNAR1 blocking antibody: MAR1-5A3 Mouse IgG1κ | BioXCell | Cat# B10241 |
| Mouse IgG1 isotype control | BioXCell | Cat# BE0083 |
| Bacterial and virus strains | ||
| Ifnb1 sgRNA CRISPR/spCas9 All-in-One Lentivector set (3 gRNA) (Mouse) | ABMGOOD | Cat# 242661140595 |
| CRISPR/spCas9 Scrambled sgRNA All-in-One Lentivector | ABMGOOD | Cat# K010 |
| Eif2ak3 sgRNA CRISPR/spCas9 All-in-One Lentivector set (3 gRNA) (Mouse) | ABMGOOD | Cat# 190761140895 |
| Biological samples | ||
| TMA slides | Moffitt Cancer Center, Biomax, TriStar Technology | Tissue Core Moffitt Cancer Center. Also, commercial vendors Biomax and TriStar Technology |
| Chemicals, peptides, and recombinant proteins | ||
| Mouse GM-CSF | Gemini | Cat# 300-308P |
| Mouse G-CSF | Gemini | Cat# 300-207P |
| APC Annexin V | BD Pharmingen | Cat# 550475; RRID: AB_2868885 |
| T-select MHC Class I mouse gp100 tetramer: KVPRNQDWL-PE | Medical & Biological Laboratories | Cat# TS-M505-1 |
| AMG-PERK-44 | Tocris | Cat# 5517 |
| PERK Inhibitor I, GSK2606414 | Sigma Aldrich | Cat# 516535-5MG |
| Thapsigargin | Sigma Aldrich | Cat# T9033-5MG |
| BMS-CCR2-22; CCR2 inhibitor | Tocris | Cat# 3129 |
| Z-Vad(OH)-FMK; Pan-Caspase inhibitor | Cayman Chemicals | Cat# 14467 |
| Ferrostatin-1; Ferroptosis inhibitor | Cayman Chemicals | Cat# 17729 |
| Necrostatin-1; Necroptosis inhibitor | Cayman Chemicals | Cat# 11658 |
| Dapansutrile; NLRP3/Pyroptosis inhibitor | Cayman Chemicals | Cat# 24671 |
| MKC-3946; IRE1a inhibitor | Cayman Chemicals | Cat# 19152 |
| Hydroxychloroquine (sulfate) | Cayman Chemicals | Cat# 17911 |
| PPADS tetrasodiun salt | Tocris | Cat# 0625 |
| Evans blue tetrasodium salt | Tocris | Cat# 0845 |
| Cycloheximide | Cayman Chemicals | Cat# 14126 |
| Fludarabine phosphate | Cayman Chemicals | Cat# 14251 |
| DNase I | Roche | Cat# 10104159001 |
| Liberase | Roche | Cat# 05401127001 |
| Ionomycin | Sigma Aldrich | Cat# I3909-1ML |
| Golgi stop | BD Biosciences | Cat# 554724; RRID: AB_2869012 |
| Perm/Wash buffer | BD Bioscience | Cat# 554723; RRID: AB_2869011 |
| Cytofix/Cytoperm | Fisher Scientific | Cat# BDB554714 |
| ACK RBC lysis buffer | Gibco | Cat# A10492-01 |
| H2DCFDA (H2-DCCF, DCF) | Invitrogen | Cat# D399 |
| 5(6)-CFDA,SE; CFSE | Invitrogen | Cat# V12883A |
| Propidium Iodide solution | BD Bioscience | Cat# 556463; RRID: AB_2869075 |
| Zombie NIR Fixable Viability Kit | Invitrogen | Cat# 423105 |
| ER-Tracker Green (BODIPY FL Glibenclamide) | Cell Signaling | Cat# 8787S |
| NIAD4 | Cayman Chemicals | Cat# 18520 |
| CRANAD2 | Cayman Chemicals | Cat# 19814 |
| Pierce Concentrator, PES, 10K MWCO 0.5ml | Thermo Scientific | Cat# 88513 |
| Mouse Cytokine Array / Chemokine Array 44-Plex (MD44) | Eve Technologies | Cat# MD44 |
| DNase I recombinant, RNAse-free | Roche | Cat# 04716728001 |
| iTaq universal SYBR green super mix | Bio Rad | Cat# 1725121 |
| Verso cDNA synthesis Kit | ThermoFisher | Cat# AB-1453/B |
| Ovalbumin peptide (OVA257-264) | Anaspec Peptide | Cat# 60193 |
| Critical commercial assays | ||
| MitoProbe DiOC2(3) Assay | Molecular Probes | Cat# M34150 |
| CellTrace Violet cell proliferation kit | ThermoFisher | Cat# C34571 |
| Anti-CD117 Microbeads, mouse | Miltenyi Biotec | Cat# 130-091-224 |
| Mojosort Streptavidin Nanobeads | Biolegend | Cat# 76447 |
| Mouse HMGB1/HMG-1 ELISA Kit (colorimetric) | Novus | Cat# NBP2-62767 |
| Mouse IFNα ELISA | Abcam | Cat# ab252352 |
| Mouse IFNβ DuoSet ELISA | R&D Systems | Cat# DY8234-05 |
| ADP/ATP Ratio assay kit | Sigma | Cat# MAK135 |
| Deposited data | ||
| RNAseq from cMoPs | GEO accession #GSE206783 https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE206783 |
GSE206783 |
| TCGA SKCM | https://www.cancer.gov/tcga | |
| Moffitt melanoma cohort | Doi: 10.1158/1055-9965.EPI-20-0307 | |
| ICI treated Melanoma cohorts | https://www.ncbi.nlm.nih.gov/bioproject/?term=PRJEB23709, https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE78220, https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE91061 | PRJEB23709, GSE78220, GSE91061 |
| Experimental models: Cell lines | ||
| B16-F10 | ATCC | CRL-6475; RRID: CVCL_0159 |
| SM1 | NA | NA |
| Lewis lung carcinoma (LLC) | ATCC | #CRL-1642; RRID: CVCL_4358 |
| Experimental models: Organisms/strains | ||
| C57BL/6 Mice | Wildtype | Envigo |
| NOD.129S7 (B6)-Rag1 tm1Mom/J | RAG1 | Jackson Laboratory |
| B6.129P2-Lyz2 tm1(Cre)Ifo/J | Lyz2-Cre | Jackson Laboratory |
| B6(Cg)-Sting1tm1.2Camb/J | STING flox/flox | Jackson Laboratory |
| B6(Cg)-Ifnar1 tm1.1Ees/J | IFNAR1 flox/flox | Jackson Laboratory |
| Eif2ak3tm1.2Drc/J | PERK flox/flox | Jackson Laboratory |
| B6(Cg)-Ifnar1tm1.2Ees/J | Total body IFNAR1 knockout | Jackson Laboratory |
| B6.129S4-CCR2 tm1lfc/J | Total body CCR2 knockout | Jackson Laboratory |
| B6.129S(Cg)-Stat1tm1Dlv/J | Total body STAT1 knockout | Jackson Laboratory |
| B6.FVB-1700016L21RikTg(Itgax-DTR/EGFP)57Lan/J | Diphtheria toxin receptor-CD11c GFP (DTR-CD11c-GFP) | Jackson Laboratory |
| B6.Cg-Gt(ROSA)26Sor tm1.4(CAG-TdTomato)Hze/J | TdTomato | Jackson Laboratory |
| B6.129-Ifnb1 tm1Lky/J | Type I interferon reporter mouse; EYFP-IFNB1 | Jackson Laboratory |
| B6.Cg-Tg(Tyr-Cre/ERT2)13Bos Braf tm1Mmcm Pten tm1Hwu/BosJ | Inducible melanoma, ERT-Tyrosinase-Cre BrafV600E Ptenfl/fl (TBP) | Jackson Laboratory |
| Oligonucleotides and Primers | ||
| Sec61b siRNA | Sigma | EMU162401 |
| Atf4 siRNA | Sigma | EMU079511 |
| Mock siRNA | Sigma | SIC001-10NMOL |
| Ccl2 Forward | IDT | CAGGTCCCTGTCATGCTTCT |
| Ccl2 Reverse | IDT | GTGGGGCGTTAACTGCATCT |
| Ccl12 Forward | IDT | ACACTGGTTCCTGACTCCTCT |
| Ccl12 Reverse | IDT | ACCTGAGGACTGATGGTGGT |
| Ifnb1 Mm_Ifnb1_1_SG | Qiagen | Cat# QT00249662 |
| Isg15 Forward | IDT | CTAGAGCTAGAGCCTGCAG |
| Isg15 Reverse | IDT | AGTTAGTCACGGACACCAG |
| Ifit3 Forward | IDT | TTCCCAGCAGCACAGAAAC |
| Ifit3 Reverse | IDT | AAATTCCAGGTCAAATGGCA |
| Cxcl10 Forward | IDT | CCAAGTGCTGCCGTCATTTTC |
| Cxcl10 Reverse | IDT | GGCTCGCAGGGATGATTTCAA |
| Actin-b | Qiagen | Cat# QT00095242 |
| Ifna1 Forward | IDT | GGATGTGACCTTCCTCAGACTC |
| Ifna1 Reverse | IDT | ACCTTCTCCTGCGGGAATCCAA |
| Ifna14 Forward | IDT | AGCAGGTAGAGATACAGGCACC |
| Ifna14 Reverse | IDT | TTTCAGGCTGGTCAGCAACTT |
| Ifna13 Forward | IDT | GGCTCAAGCCATCCCTTTTG |
| Ifna13 Reverse | IDT | TGAAACATGTAGGCAGGTTGATTG |
| Ifna7 Forward | IDT | GTCCTGGTGGTGTTGAGCTA |
| Ifna7 Reverse | IDT | TGCAGAACACAGAGGGCTTG |
| Ifna16 Forward | IDT | AGGATGTGACCTGCCTCAGACT |
| Ifna16 Reverse | IDT | AGGGTATCCACCTTCTCCTGGG |
| Atf4 primers | Qiagen | PPM04670E-200 |
| Sec61b Forward SET1 | IDT | GATTCCCCAGGGCTCAAGT |
| Sec61b Reverse SET1 | IDT | AGCCCAATCTATGATCGCGT |
| Sec61b Forward SET2 | IDT | CCCAGTGCTGGTGATGAGTC |
| Sec61b Reverse SET2 | IDT | GCCCAATCTATGATCGCGTG |
| Software and algorithms | ||
| Prism 7 | GraphPad | |
| FlowJo | FlowJo | |
| Wave | Agilent Technologies | |
| Aperio Image scope | Leica Biosystems | |
| Zen 2.3 (blue edition) | Carl Zeiss AG | |
| Definiens Tissue Studio software v4.7 | Definiens AG | |
| Halo | Indica labs | |
| Other | ||