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. 2022 Sep 30;13:985223. doi: 10.3389/fphar.2022.985223

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Copyright © 2022 Quan, Yin, Chen, Lang, Han, Yi, Zhang, Yue, Tian, Chen, Du, Wang, Dai, Hua, Zeng, Li and Zhao.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The effect of BLM on the expression of pro-apoptotic key proteins in lung tissue of mice and the quantitative results of TUNEL apoptosis assay. (A) IHC schematic diagram. (B) IHC staining of cleaved caspase-3 in lung tissue (×400). (C) IHC staining of BAX in lung tissue (×400). (D) Examples of color deconvolution analysis. (E) Quantitative results of cleaved caspase-3 in lung tissue. (F) Quantitative results of BAX in lung tissue. (G) Quantitative results of TUNEL apoptosis in lung tissue. ***p < 0.001 vs. normal and sham group. “ns” indicates not significant.