Table 3.

Selected proteins, antibodies, dilutions.

Target protein	Primary antibody (COMPANY, City, Country)	Dilution/incubation	Secondary antibody (COMPANY, City, Country)	Dilution/incubation
TPH2	Rabbit anti-TPH2 LS‑C346253, Lifespan Bio, Seattle, WA, USA	1:500 in 5% skim milk/ overnight	Scanlater Eu-labeled Goat Anti- Rabbit lgG R8029 Molecular Devices, Sunnyvale, CA, USA	1:5000 in 5% skim milk/ RT 1 h
DBH	Rabbit anti-DBH b209487, Abcam, Cambridge, UK	1:500 in 5% skim milk/ overnight	Scanlater Eu-labeled Goat Anti-Rabbit lgG R8029 Molecular Devices, Sunnyvale, CA, USA	1:5000 in 5% skim milk/ RT 1 h
NET	Rabbit anti-NET LS-C408311, Lifespan Bio, Seattle, WA, USA	1:1000 in 5% skim milk/ overnight	Scanlater Eu-labeled Goat Anti- Rabbit lgG R8029 Molecular Devices, Sunnyvale, CA, USA	1:5000 in 5% skim milk/ RT 1 h
SERT	Rabbit anti-SERT ab9726, Sigma Aldrich, Saint Louis, MO, USA	1:500 in 5% skim milk/ overnight	Scanlater Eu-labeled Goat Anti-Rabbit lgG R8029 Molecular Devices, Sunnyvale, CA, USA	1:5000 in 5% skim milk/ RT 1 h
5HT1B	Rabbit anti-5HT1B SAB4501471, Sigma Aldrich, Saint Louis, MO, USA	1:500 in 5% skim milk/ overnight	Scanlater Eu-labeled Goat Anti-Rabbit lgG R8029 Molecular Devices, Sunnyvale, CA, USA	1:5000 in 5% skim milk/ RT 1 h
GLP1R	Rabbit anti-GLP1R PA5–70645, Invitrogen, Waltham, MA, USA	1:500 in 5% skim milk/ overnight	Scanlater Eu-labeled Goat Anti-Rabbit lgG R8029 Molecular Devices, Sunnyvale, CA, USA	1:5000 in 5% skim milk/ RT 1 h
β-actin	Mouse anti-β-actin #3700, Cell Signaling, Danvers, MA, USA	1:1000 in 5% skim milk/ overnight	Scanlater Eu-labeled Goat Anti-Mouse lgG R8028 Molecular Devices, Sunnyvale, CA, USA	1:5000 in 5% skim milk/ RT 1 h

Description of the selected proteins as well as primary/secondary antibody, dilutions and incubation times used. Tyrosine Hydroxylase Isoforms 2 (TPH2), Dopamineβ Hydroxylase (DBH), Norepinephrine Transporter (NET), Serotonin Transporter (SERT), 5-hydroxytryptamine receptor 1B (5HT1B), Glucagon-like peptide 1 receptor (GLP1R).