Fig. 5.

Mangiferin reduced MTX-induced renal inflammation. Inflammatory mediators were measured in the kidney of rats pretreated with MF or MF + BADGE then received MTX. (A) Relative mRNA expression of NFκB was measured by qRT-PCR. (B-D) Total (tNFκB), phosphor (pNFκB), IL-1β and TNF-α protein expression was measured using ELISA techniques, respectively. (E) Relative mRNA expression of NFκB was measured by qRT-PCR. Data is represented as (Mean ± SEM), N ≥ 4, * p ≤ 0.05 compared to control group, ^#p ≤ 0.05 compared to MTX group, ^$p ≤ 0.05 compared to MF group.