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. 2022 Sep 8;17(10):2286–2302. doi: 10.1016/j.stemcr.2022.08.003

Figure 2.

Figure 2

iPSC-HLCs and PHH are susceptible to EBOV infection

(A) Immunofluorescence analysis of EBOV-infected Huh7 cells, BU2 iPSC-HLCs, and donor A PHHs. Cells were mock infected or infected with EBOV at an MOI of 10, fixed at the indicated time points, and stained with an antibody directed against EBOV nucleoprotein (red). Cell nuclei were stained with DAPI. Images are representative of triplicate replicates (n = 3 technical replicates per cell type). Images of iPSC-HLCs and Huh7 cells taken at 40× magnification. Images of PHHs taken at 20× magnification. Scale bar represents 20 μm.

(B) Higher magnification to visualize viral inclusions. Images taken at 63× magnification. Scale bar represents 20 μm.

(C) Viral titers of each EBOV-infected hepatocyte platform at the indicated time points. Bars represent the average of two technical replicates (n = 2 technical replicates per cell type). iPSC-HLCs, BU2; PHH, donor A.