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. 2022 Sep 8;17(10):2286–2302. doi: 10.1016/j.stemcr.2022.08.003

Figure 4.

Figure 4

Upregulation of ISGs and downregulation of genes involved in liver function in EBOV-infected iPSC-HLCs at 2 and 3 dpi

(A) Principal component analysis (PCA) of transcriptional responses of EBOV- and mock-infected BU3 iPSC-HLCs at each time point (n = 2 technical replicates per infection condition per time point). PCA1 represents the majority of variance and is associated with the day of differentiation. PCA2 represents the second largest contributor of variance and is associated with infection status.

(B) Heatmap displaying the 250 most variable genes at each time point. Highlighted are the top 30 upregulated and downregulated DEG at 3 dpi. Key for the value of the logFC to the right of the plot.

(C) Heatmap displaying the logFC for a list of ISGs (right of the plot) of EBOV-infected iPSC-HLCs compared with mock-infected cells at the indicated time points. Key logFC change in expression shown to the right.

(D) Heatmap displaying the logFC for a list of genes involved in liver function (right of the plot) of mock- and EBOV-infected iPSC-HLCs at 3 dpi. Shown are the technical replicates for each condition. Key logFC change in expression shown to the right.