Skip to main content
. 2022 Sep 8;17(10):2286–2302. doi: 10.1016/j.stemcr.2022.08.003

Figure 6.

Figure 6

RNA-FISH analysis reveals expression of IFNB1 and CXCL10 in uninfected bystander cells and cells with weak EBOV mRNA signal intensity

(A) EBOV-infected BU1 and BU2 iPSC-HLCs were fixed at the indicated times and analyzed by RNA-FISH using a combination of probes against EBOV VP35 mRNA (green) and CXCL10 (magenta, top row), or IFNB1 (IFNβ, magenta, bottom row) mRNA. Shown are representative images from both donors at each time point at 20× magnification, and right column shows inset region of merged images (n = 2 technical replicates per iPSC donor per time point for a total of four replicates per time point; two technical replicates of two different donors). Scale bars represent 100 μm.

(B–D) Quantification of gene expression from images using at least 1,200 cells and eight fields of view per time point and probe combination. (B) Percentage of total cells positive for EBOV VP35 mRNA at each time point. Error bars represent SD. (C) Percentage of infected cells expressing CXCL10 mRNA, left axis, and percentage of uninfected cells expressing CXCL10 mRNA, right axis. Error bars represent SD. (D) Percentage of infected cells expressing IFNB1 mRNA, left axis, and percentage of uninfected cells expressing IFNB1 mRNA, right axis. Error bars represent SD. (A) Upper, BU2; lower, BU2. (B) Upper, BU1; lower, BU1. (C) Upper, BU1; lower, BU1.