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. 2022 Sep 26;50(18):10544–10561. doi: 10.1093/nar/gkac798

Figure 1.

Figure 1.

Drought inhibits lateral root development by inhibiting auxin signaling. (A) Root morphologies of Col-0 plants grown vertically on MS plates containing with or without 75 mM mannitol. Four-day-old plants grown on MS medium were transferred to the plates shown. The plants were photographed at 7 d after the transfer. The scale bar represents 1 cm. (B) Primary root (PR) length and density of lateral root primordia (LRP), emerged lateral roots (LRE), and total lateral roots (LRP + LRE) of Col-0 plants grown vertically on MS plates as shown in Supplementary Figure S1. The bars indicate the mean ± S.D. (n = 20). Different letters represent significant differences (P < 0.001). (C) Transcriptional inhibition of LBD genes by treatment with mannitol. Transcript levels of LBD16 and LBD29 were measured by qPCR using gene-specific primers. The bars indicate the mean ± S.D. (n = 3). Different letters represent significant differences determined with Student's t-test (P < 0.01). (D) Mannitol inhibits auxin signaling through MPK cascade. DR5::GUS plants were treated with 200 mM mannitol alone or in combination with 10 μM U0126 for 6 h. GUS activities were detected in the lateral root primordia and emerged lateral root. The scale bars represent 50 μm.